Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and can be used as natural sugar substitutes due to their far higher sweetening power than normal sucrose or glucose. The sweetness is estimated to be about 400 times higher. This application describes an easy transfer of an existing analytical HPLC method to a preparative HPLC using overload experiments together with the KNAUER Scale-Up converter. Furthermore, this approach of scale-up is also applicable for different analytes and thus provides a fast scale-up.
A - Transfer gradient to isocratic method; 1) rebaudioside A, 2) stevioside, 20 μL, C18, 5 μm particle; B - Overlay 100 μL (blue) and 200 μL (red) injection, C18, 10 µm particle
Overload experiments on preparative column, 200 μL (red), 500 μL (blue), 2000μL (green); 1) rebaudioside A, 2) stevioside, blue bars – matrix, 25°C, 22 ml/min
|Substances||rebaudioside A, stevioside|
|Key words||Stevia, sweetener, steviol glycosides, online SPE, solid phase extraction, purification, preparative HPLC, sample preparation|
|CAS number||58543-16-1, 57817-89-7|
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