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Scale-Up of an analytical HPLC method for steviol glycosides to a preparative approach

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Application No.: VFD0170

Scale-Up of an analytical HPLC method for steviol glycosides to a preparative approach

Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and can be used as natural sugar substitutes due to their far higher sweetening power than normal sucrose or glucose. The sweetness is estimated to be about 400 times higher. This application describes an easy transfer of an existing analytical HPLC method to a preparative HPLC using overload experiments together with the KNAUER Scale-Up converter. Furthermore, this approach of scale-up is also applicable for different analytes and thus provides a fast scale-up.

Technical Data

Application details

Method HPLC
Mode RP
Substances rebaudioside A, stevioside
Key words Stevia, sweetener, steviol glycosides, online SPE, solid phase extraction, purification, preparative HPLC, sample preparation
CAS number 58543-16-1, 57817-89-7

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