A stable column temperature is crucial for reproducible separations and constant retention times. Moreover, increasing the column temperature leads to a decreased solvent viscosity and therewith to a lower backpressure. Consequently, flow rates can be raised resulting in faster analysis times of your HPLC system. KNAUER`s column tempering solutions improve your separations.
Thermostats and column heating sleeves provide constant and evenly distributed temperatures in HPLC columns for reproducible separations
Column Thermostat CT 2.1
Controlling column temperature
Column thermostats are an essential part of each HPLC system since most HPLC separation parameters are temperature-sensitive. Hence, the control of the column temperature is one of the most important prerequisites for reproducible results in HPLC.
Rule of thumb
For flow rates higher than 500 µl/min or temperatures above 50 °C, the use of a pre-heating cartridge is recommended.
The basic column thermostat is a software controlled heating department, designed for up to 8 columns depending on the size of the columns. Suitable for analytical und semipreparative columns in the peltier element controlled temperature range of 5–85 °C. Can be additionally equipped with a preheating cartridge.
Columns with an ID > 16 mm should be heated by other options, because the big inner diameter and the higher flowrate can lead to temperature profiles inside the column which can negativly influence the analytical performance.
Pre-heating cartridges or eluent heaters can be used to warm up the eluent before it reaches the column. This helps to stop the formation of temperature profiles inside the column.
While pre-heating cartridges are built in the column oven and mainly used in analytical HPLC, eluent heaters are stand-alone devices and are used for preparative HPLC.
Another solution is the pump head heating devices, which directly heats the solvent inside the pump head to start the heating process even earlier in the HPLC and achieve a constant temperature.
Options for Pre-Column tempering
Pump head tempering
Pre-heating cartridges can be installed inside the column oven or be remotly placed inside another heating element. Due to the internal volume and geometry the solvent is heated at the desired temperature. This avoids temperature profiles inside the column. Depending on temperature and solvent flow, a heating cartridge is recommended.
The inner diameter should be similar to capillaries used in the system to avoid any unnecessary volume contraction.
KNAUER offers different types of eluent heaters according to your needs. A 1-channel eluent heater is used only for heating the eluent. A 2-channel eluent heater offers the options for heating the eluent, controling a column heating sleeve and comes with two external temperature sensors to enable an additional temperature control.
The specially designed pump head cooling and heating device A2034-1 can be used for 100/250/500/1000 ml pump heads.
With the help of an internal circulating water bath, the pump head can be heated or cooled.
The connection of a circulating water bath to the thermostatization device is easily made by using the supplied silicon tubings.
Column heating sleeves
Our column heating Sleeves are the perfect solution for thermosetting your preparative column hardware. Regardless if you want to reduce viscosity or improve performance, the column sleeves are a very compact and cost-effective solution for your preparative column heating tasks.
They are available for all preparative KNAUER column dimensions up to a diameter of 101mm, a length of 1000mm, and up to 100 °C.
Several options are available to control the temperature of the sleeves.
Standalone options would include the A57024 as a separate temperature controller. As for software approaches the sleeves can also be controlled via the PurityChrom software together with a Eluent heater.
Our column heating sleeves are available as a normal variant and a moisture-proof variant.
Do you need to heat columns with different dimensions?
No problem, we can offer customized column sleeves (A57032).
Column heating sleeves
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