The high-quality FPLC column alternative to reach your purification goals KNAUER Sepapure FPLC columns

The Sepapure® FPLC column line offers high-quality alternatives for Size-Exclusion (SEC), Affinity (AC) and Ion-Exchange chromatography (IEX). The FPLC column line is completing the KNAUER FPLC portfolio and provides the best LC column addition to your FPLC system.

The FPLC columns come as prepacked ready-to-use 5 ml cartridge and can also be purchased as bulk resin. The media for affinity chromatography as well as ion-exchange chromatography is based on agarose, which is very proven in FPLC chromatography.
For affinity chromatography the agarose solid phase is functionalized with ligands for purification of recombinantly expressed proteins with His- or GST-tags or antibodies via Protein A or Protein G affinity. The Ion-Exchange columns can be purchased as weak and strong anion or cation exchangers. The basis of our FPLC desalting column is dextran, which reliably separates small molecules and salts from valuable proteins.

With these materials, the Sepapure FPLC columns are the best matching biochromatography columns to your precious AZURA FPLC system, giving you the complete and reliable KNAUER FPLC package.

Unhappy with your current FPLC column? Change today to KNAUER Sepapure FPLC columns!

Is your FPLC column too expensive? Your FPLC column manufacturer does not provide the support you need?
Change today to KNAUER FPLC columns, the high quality alternative - Made in Germany. We provide great support and a complete FPLC solution designed for your application.

FPLC column based on Agarose or Dextran

Made in Germany

Complete FPLC solution from one supplier

For Size-Exclusion (SEC), Affinity (AC) and Ion-Exchange chromatography (IEX)

That's what the users say

"I performed a lot of protein purification, like AC, IEX and SEC. During my applications I compared the new FPLC column line Sepapure to other vendor s columns. I am extremely happy with the quality of KNAUER FPLC columns. Sepapure is a very good alternative, I can totally recommend it to any lab."

Dr. Ulrike Krop, FPLC Application Specialist

See comparison

Affinity Chromatography (AC)

Antibody affinity: Protein A and Protein G
Tag affinity: His-tag

Antibody affinity: Protein A and Protein G

For the purification of antibodies and antibody fragments, Sepapure® AC media are available functionalized with Protein A and Protein G.

Article number	Name	Dimension	Application	Particle size	Maximum pressure
010X40USPZ	Sepapure Protein A FF 1 ml	Prepacked 1 ml	for antibody purification	100 µm	3 bar
020X40USPZ	Sepapure Protein A FF 5 ml	Prepacked 5 ml	for antibody purification	100 µm	3 bar
010X40VSPZ	Sepapure Protein G FF 1 ml	Prepacked 1 ml	for antibody purification	100 µm	3 bar
020X40VSPZ	Sepapure Protein G FF 5 ml	Prepacked 5 ml	for antibody purification	100 µm	3 bar

All media are also available as bulk.

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Tag affinity: His-tag

For the purification of biomolecules through interaction with a recombinant tag, KNAUER offers Sepapure® media for purifying histidine- tagged proteins.

Article number	Name	Dimension	Application	Particle size	Maximum pressure
010X39FPSZ	Sepapure FF Ni-NTA 1 ml	Prepacked 1 ml	for His-tag purification	100 µm	3 bar
020X39FSPZ	Sepapure FF Ni-NTA 5 ml	Prepacked 5 ml	for His-tag purification	100 µm	3 bar

All media are also available as bulk.

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Size exclusion chromatography (SEC)

Size exclusion chromatography (SEC), separates molecules on the basis of size differences.

KNAUER offers desalting columns for group separations of large molecular weight biomolecules from small molecular weight compounds.

Article number	Name	Dimension	Application	Particle size	Maximum pressure	Molecular weight range
040X460SPZ	Sepapure Desalting 5 ml	Prepacked 5 ml	desalting	20 - 50 µm	3 bar	1 - 5 kDa

All media are also available as bulk.

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Ion Exchange Chromatography (IEX)

Ion exchange (IEX) chromatography can separate molecules or groups of molecules according to their charge at a specific pH. Separation is based on the reversible interaction between a charged molecule and the oppositely charged chromatography medium.

Sepapure® media are available in weak and strong cation and anion exchange modifications.

Article number	Name	Dimension	Application	Particle size	Maximum pressure
010X15ISPZ	Sepapure DEAE FF6 1 ml	Prepacked 1 ml	weak anion exchange	100 µm	3 bar
020X15ISPZ	Sepapure DEAE FF6 5 ml	Prepacked 5 ml	weak anion exchange	100 µm	3 bar
010X15HSPZ	Sepapure Q FF6 1 ml	Prepacked 1 ml	strong anion exchange	100 µm	3 bar
020X15HSPZ	Sepapure Q FF6 5 ml	Prepacked 5 ml	strong anion exchange	100 µm	3 bar
010X15QSPZ	Sepapure CM FF6 1 ml	Prepacked 1 ml	weak anion exchange	100 µm	3 bar
020X15QSPZ	Sepapure CM FF6 5 ml	Prepacked 5 ml	weak cation exchange	100 µm	3 bar
010X15RSPZ	Sepapure SP FF6 1 ml	Prepacked 1 ml	strong anion exchange	100 µm	3 bar
020X15RSPZ	Sepapure SP FF6 5 ml	Prepacked 5 ml	strong cation exchange	100 µm	3 bar