Sepapure® FPLC columns and resins for all common protein purification tasks: Size Exclusion Chromatography (SEC), Affinity Chromatography (AC) and Ion-Exchange Chromatography (IEX)
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FPLC columns based on Agarose or Dextran
Made in Germany
Complete FPLC solution from one supplier
For Size-Exclusion (SEC), Affinity (AC) and Ion-Exchange chromatography (IEX)
"I performed a lot of protein purification, like AC, IEX and SEC. During my applications I compared the new FPLC column line Sepapure to other vendor s columns. I am extremely happy with the quality of KNAUER FPLC columns. Sepapure is a very good alternative, I can totally recommend it to any lab."
Dr. Ulrike Krop, FPLC Application Specialist
See comparisonFor the purification of antibodies and antibody fragments, Sepapure® AC media are available functionalized with Protein A and Protein G.
| Article number | Name | Dimension | Application | Particle size | Maximum pressure |
|---|---|---|---|---|---|
| 010X40USPZ | Sepapure Protein A FF 1 ml | Prepacked 1 ml | for antibody purification | 100 µm | 3 bar |
| 020X40USPZ | Sepapure Protein A FF 5 ml | Prepacked 5 ml | for antibody purification | 100 µm | 3 bar |
| 010X40VSPZ | Sepapure Protein G FF 1 ml | Prepacked 1 ml | for antibody purification | 100 µm | 3 bar |
| 020X40VSPZ | Sepapure Protein G FF 5 ml | Prepacked 5 ml | for antibody purification | 100 µm | 3 bar |
All media are also available as bulk.
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For the purification of biomolecules through interaction with a recombinant tag, KNAUER offers Sepapure® media for purifying histidine- tagged proteins.
| Article number | Name | Dimension | Application | Particle size | Maximum pressure |
|---|---|---|---|---|---|
| 010X39FPSZ | Sepapure FF Ni-NTA 1 ml | Prepacked 1 ml | for His-tag purification | 100 µm | 3 bar |
| 020X39FSPZ | Sepapure FF Ni-NTA 5 ml | Prepacked 5 ml | for His-tag purification | 100 µm | 3 bar |
All media are also available as bulk.
ContactSize exclusion chromatography (SEC), separates molecules on the basis of size differences.
KNAUER offers desalting columns for group separations of large molecular weight biomolecules from small molecular weight compounds.
| Article number | Name | Dimension | Application | Particle size | Maximum pressure | Molecular weight range |
|---|---|---|---|---|---|---|
| 040X460SPZ | Sepapure Desalting 5 ml | Prepacked 5 ml | desalting | 20 - 50 µm | 3 bar | 1 - 5 kDa |
All media are also available as bulk.
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Ion exchange (IEX) chromatography can separate molecules or groups of molecules according to their charge at a specific pH. Separation is based on the reversible interaction between a charged molecule and the oppositely charged chromatography medium.
Sepapure® media are available in weak and strong cation and anion exchange modifications.
| Article number | Name | Dimension | Application | Particle size | Maximum pressure |
|---|---|---|---|---|---|
| 010X15ISPZ | Sepapure DEAE FF6 1 ml | Prepacked 1 ml | weak anion exchange | 100 µm | 3 bar |
| 020X15ISPZ | Sepapure DEAE FF6 5 ml | Prepacked 5 ml | weak anion exchange | 100 µm | 3 bar |
| 010X15HSPZ | Sepapure Q FF6 1 ml | Prepacked 1 ml | strong anion exchange | 100 µm | 3 bar |
| 020X15HSPZ | Sepapure Q FF6 5 ml | Prepacked 5 ml | strong anion exchange | 100 µm | 3 bar |
| 010X15QSPZ | Sepapure CM FF6 1 ml | Prepacked 1 ml | weak anion exchange | 100 µm | 3 bar |
| 020X15QSPZ | Sepapure CM FF6 5 ml | Prepacked 5 ml | weak cation exchange | 100 µm | 3 bar |
| 010X15RSPZ | Sepapure SP FF6 1 ml | Prepacked 1 ml | strong anion exchange | 100 µm | 3 bar |
| 020X15RSPZ | Sepapure SP FF6 5 ml | Prepacked 5 ml | strong cation exchange | 100 µm | 3 bar |
All media are also available as bulk.
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Is your FPLC column too expensive? Your FPLC column manufacturer does not provide the support you need?
Change today to KNAUER FPLC columns, the high quality alternative - Made in Germany. We provide great support and a complete FPLC solution designed for your application.
Kit consisting of Sepapure FF Ni-NTA (5 ml) and Sepapure Desalting (5 ml) column
The complete system solution for FPLC on a minimum footprint. The flexible protein purification platform can be configured according to your application needs.
PurityChrom® is a volume-based chromatography software especially designed for the area of bio purification and FPLC. .
Choose between a variaty of column holders, clamps and thermostatting units to organize your lab.
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