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Prepacked FPLC columns for protein purification
The high-quality alternative FPLC column to reach your purification goals 

KNAUER is introducing its new Sepapure® FPLC columns 

The new Sepapure® FPLC column product line offers new high-quality alternatives for Size-Exclusion (SEC), Affinity (AC) and Ion-Exchange chromatography (IEX). The  FPLC column line is completing the KNAUER FPLC portfolio and provides the best LC column addition to your FPLC system.

The FPLC columns come as prepacked ready-to-use 1 ml or 5 ml cartridge and can also be purchased as bulk resin. The media for affinity chromatography as well as ion-exchange chromatography is based on agarose, which is a well-known in FPLC chromatography. The stationary phase or affinity chromatography the agarose solid phase is functionalized with ligands for purification of recombinantly expressed proteins with His- or GST-tags or antibodies via Protein A or Protein G affinity. The Ion-Exchange columns can be purchased as weak and strong anion or cation exchangers. The basis of our FPLC desalting column is dextran, which reliably separates small molecules and salts from valuable proteins.

With these materials, the FPLC column Sepapure is providing the best matching columns to your precious AZURA FPLC system, giving you the complete and reliable KNAUER FPLC package.

That's what the users say

  • "I performed a lot of protein purification, like AC, IEX and SEC. During my applications I compared the new FPLC column line Sepapure to other vendor s columns.  I am extremely happy with the quality of KNAUER FPLC columns. Sepapure is a very good alternative, I can totally recommend it to any lab."

    Dr. Ulrike Krop, FPLC Application Specialist

    See comparison

Which is your application area?

Affinity Chromatography (AC)

  • Antibody affinity: Protein A and Protein G
  • Tag affinity: GST- and His-tag
  • Antibody affinity: Protein A and Protein G

    For the purification of antibodies and antibody fragments, Sepapure® AC media are available functionalized with Protein A and Protein G.

     

    Article number Name Dimension Application Particle size Maximum pressure
    010X40USPZ Sepapure Protein A FF 1 ml Prepacked 1 ml for antibody purification 100 µm 3 bar
    020X40USPZ Sepapure Protein A FF 5 ml Prepacked 5 ml for antibody purification 100 µm 3 bar
    010X40VSPZ Sepapure Protein G FF 1 ml Prepacked 1 ml for antibody purification 100 µm 3 bar
    020X40VSPZ Sepapure Protein G FF 5 ml Prepacked 5 ml for antibody purification 100 µm 3 bar

    All media are also available as bulk. 

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  • Tag affinity: GST- and His-tag

    For the purification of biomolecules through interaction with a recombinant tag, KNAUER offers Sepapure® media for purifying histidine- or GST-tagged proteins. 

     

    Article number Name Dimension Application Particle size Maximum pressure
    010X39FPSZ Sepapure FF Ni-NTA 1 ml Prepacked 1 ml for His-tag purification 100 µm 3 bar
    020X39FSPZ Sepapure  FF Ni-NTA 5 ml Prepacked 5 ml for His-tag purification 100 µm 3 bar
    010X39PSPZ Sepapure FF4 Glutathione 1 ml Prepacked 1 ml for GST-tag purification 100 µm 3 bar
    020X39PSPZ Sepapure FF4 Glutathione 5ml Prepacked 5 ml for GST-tag purification 100 µm 3 bar

    All media are also available as bulk. 

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Size exclusion chromatography (SEC) 

  • Size exclusion chromatography (SEC), separates molecules on the basis of size differences.

    KNAUER offers desalting columns for group separations of large molecular weight biomolecules from small molecular weight compounds.

     

    Article number Name Dimension Application Particle size Maximum pressure Molecular weight range
    010X460SPZ Sepapure Desalting 1 ml Prepacked 1 ml desalting 20 - 50 µm 3 bar 1 - 5 kDa
    020X460SPZ Sepapure Desalting 5 ml Prepacked 5 ml desalting 20 - 50 µm 3 bar 1 - 5 kDa

    All media are also available as bulk.

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Ion Exchange Chromatography (IEC)

  • Ion exchange (IEC) chromatography can separate molecules or groups of molecules according to their charge at a specific pH. Separation is based on the reversible interaction between a charged molecule and the oppositely charged chromatography medium. 

    Sepapure® media are available in weak and strong cation and anion exchange modifications.

     

    Article number Name Dimension Application Particle size Maximum pressure
    010X15ISPZ Sepapure DEAE FF6 1 ml Prepacked 1 ml weak anion exchange 100 µm 3 bar
    020X15ISPZ Sepapure DEAE FF6 5 ml Prepacked 5 ml weak anion exchange 100 µm 3 bar
    010X15HSPZ Sepapure Q FF6 1 ml Prepacked 1 ml strong anion exchange 100 µm 3 bar
    020X15HSPZ Sepapure Q FF6 5 ml Prepacked 5 ml strong anion exchange 100 µm 3 bar
    010X15QSPZ Sepapure CM FF6 1 ml Prepacked 1 ml weak cation exchange 100 µm 3 bar
    020X15QSPZ Sepapure CM FF6 5 ml Prepacked 5 ml weak cation exchange 100 µm 3 bar
    010X15RSPZ Sepapure SP FF6 1 ml Prepacked 1 ml strong cation exchange 100 µm 3 bar
    020X15RSPZ Sepapure SP FF6 5 ml Prepacked 5 ml strong cation exchange 100 µm 3 bar

    All media are also available as bulk. 

    Contact

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