What is Fast Protein Liquid Chromatography (FPLC)?
FPLC is a form of liquid chromatography to purify large biomolecules like proteins or DNA. External factors like high temperature, high pressure, extreme pH, or solvents can disturb the protein structure and are therefore avoided in FPLC. Besides, the method uses column materials out of agarose or polymer material which are very sensitive against pressure fluctuations and air bubbles.
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