Systems for FPLC

The flexible protein purification platform

Complete solutions for FPLC on a minimum footprint: AZURA® FPLC systems combine flexibility and reliability. The biocompatible/metal free AZURA® FPLC is the perfect choice for your protein purification task. 
Design your AZURA Bio purification system to your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection as well as fraction collection enable the user to automate the purification process.
A large range of different detectors make your target molecules visible. Different flow rates and compatibility to columns from all venders offer maximum flexibility. The intuitive software PurityChrom® combines all the advantages of a versatile purification software.

What is Fast Protein Liquid Chromatography (FPLC)?

FPLC is a form of liquid chromatography to purify large biomolecules like proteins or DNA. External factors like high temperature, high pressure, extreme pH, or solvents can disturb the protein structure and are therefore avoided in FPLC. Besides, the method uses column materials out of agarose or polymer material which are very sensitive against pressure fluctuations and air bubbles.
We designed our systems to meet your purification challenges!

Configure your perfect FPLC system

Build a custom solution based on your requirements. Just pick the components that match your method parameters.

From simple to complex, from Lab to Pilot scale: Design your AZURA® FPLC system according to your purification task!
AZURA Bio Lab allows you to create FPLC systems with highest independence. Just pick your modules and build-up the system yourself. Continue flexibility with intuitive PurityChrom® software.

AZURA® Bio purification: You choose the method

  • Size Exclusion (SEC)
  • Affinity (AC)
  • Ion-Exchange (IEX)
  • Hydrophobic Interaction (HIC)
  • Size Exclusion Chromatography (SEC)

    Separate according to size. AZURA Compact SEC systems take over time-consuming SEC methods in your lab without blocking your valuable FPLC system. Thanks to its compact design and intuitive FPLC software PurityChrom®, the system offers outstanding performance and ease of use. Pre-designed methods are included in the software and can be easily adapted by changing the column volume. AZURA Compact SEC supports all columns available on the market.

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  • Affinity Chromatography (AC)

    Specific binding of protein of interest. The AZURA Compact AC system qualifies for fast and reliable affinity chromatography. Select your sample, your washing and elution buffer using the selection valve. Your proteins of interest are detected by UV and automatically collected via the fractionation valve. 

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  • Ion-Exchange Chromatography (IEX)

    Separation takes place according to the charge of the protein and gradient elution.

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  • Hydrophobic Interaction Chromatography (HIC)

    Separation is performed based on hydrophobic interaction and gradient elution.

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Purification strategy

Often a sequence of different methods is used in purification.


Normally a combination of methods is used in protein purification.
• The “capture” step purifies the protein from the crude extract.
• The “intermediate” step removes further contamination.
• The aim of the final “polishing” step is to get rid of all remaining impurities in order to gain a highly purified product.

Scale-up from Lab to Pilot

Choose the Pilot series if you want to increase your productivity even more. Upscale our Lab configuration with same flexibility, software PurityChrom® but minimal footprint. Just transfer and upscale your methods. Flow rates up to 1000 ml/min and loads up to several grams are possible.

FPLC Software PurityChrom®

The PurityChrom® is a powerful software to control your FPLC system. Get familiar with PurityChrom® in shortest time and with no effort due to the intuitive and clearly structured user interface. Choose a time- or volume based workflow by just clicking one button. Create methods with highest flexibility to realize complex application without losing easy handling. Offline licenses for creating methods and data evaluation are for free.

Bio purification accessories and FPLC spare parts

Sometimes it is the little things that produce the big results. In addition to systems and devices, KNAUER offers a wide range of Accessories and Supplies. These parts are compatible with all commonly used HPLC or FPLC systems, columns, pumps, as well as detectors and were designed to simplify everyday laboratory life.

Accessory Features Benefit
• Contains two pressure sensors
• Automatic determination of pressure difference with PurityChrom®
• Connect 1/16” or 1/8” tubings
• Up to 250 ml/min and 60 bar
Monitor pressure over the
column bed and protect
column from damage
Air Sensor • Detect end of buffer or end of sample with PurityChrom®
• Up to four air sensors per system
• For transparent tubings with 1/16” or 1/8” or 1/4” outer diameter
Protect column from air
damage and support
automation ( e.g. sample
• Attach air sensor, pressure control, AZURA Organizer or your
interface box to the side panel of your AZURA L device
Organize your system.
• Attach columns from 5 mm to 26 mm diameter, falcon tubes, a
back pressure regulator or a pH flow cell
Organize accessories directly
at the system and reduce
dead volume
Back pressure
regulator (BPR)
• Apply a constant back pressure to your system
• Freely adjustable between 1-20 bar or 20-103 bar
Prevent formation of air
bubbles after the column
which disturb detector signal

AZURA® Bio purification (FPLC)

The complete brochure gives you an excellent overview of protein purification with the flexible AZURA FPLC systems. Get to know the components of the FPLC Bio Systems and the different possibilities in detail.
We have designed our systems to meet your purification challenges!

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