Simulated moving bed chromatography (SMBC) is increasingly applied as a separation technique in the pharmaceutical industry, production of fine chemicals, and in the field of bioengineering.
SMB is a method in process chromatography that enables substance mixtures to be continuously separated and extracted in two fractions. Its efficiency is significantly higher than batch chromatography, through better utilization of the column stationary phase.
Although in principle limited to two fraction separations, each partial fraction can be separated into a further fraction by repeated use of the SMB process – down to binary substance mixtures.
Typically, the SMB process is used for two component mixtures, like chiral separations or mixtures with a "leading" or a "trailing" key product peak in the chromatogram.
Due to their modular design, AZURA SMB systems can also be configured for other multi-column processes. KNAUER offers SMBC systems for purification up to 1000 kg/year.
THE SMB PRINCIPLE
Other systems might be limited regarding their batch program adaption (wash+elution time ≤ capture time), due to the flexible column distribution the length of every zone can be adjusted, even if there is a critical change in the feed concentration.
The separation of biomolecules can also be done with 2, 3 or even 4 columns. But these systems are often very limited and cannot handle typical problems regarding process stability and flexibility as easy as the AZURA® SMB system can do. The 8 column setup has many advantages.
Sometimes additional purification steps are necessary to regenerate the column to keep their lifetime and potential at a maximum. Two and three columns systems often cannot handle this problem easily.
More columns mean longer system run time and less down time. You will have up to four times less downtime due to column change. Use your working time most efficient.
AZURA® SMB Lab System
The AZURA® Lab SMB System is optimized for the separation of binary mixtures in gram-scale. As with the AZURA Lab SMB System special emphasis is put on the continuous operation mode and high productivity. The SMB standard configuration consists of four AZURA Assistans ASM 2.1L with seven multiposition valves and four P 4.1S Pumps as well as our user friendly software PurityChrom MCC and the required IT hardware. Referring to the special needs of every separation the SMB system can be freely configured (e.g. closed-loop, open-loop,) and is upgradable (e.g. detector, flow meter). A maximum pressure up to 400 bar (six columns, 10 ml/min). Depending on your application the columns can be heated or cooled to ensure the perfect and most constant separation conditions. It can be chosen between a stainless steel or a bioinert version.Read more
AZURA® SMB Pilot System
The AZURA Pilot SMB System is optimized for the separation of binary mixtures in gram to kilogram-scale. As with the AZURA Pilot SMB System special emphasis is put on the continuous operation mode and high productivity. The SMB standard configuration consists of four Pump 2.1L and seven multiposition valves integrated into four AZURA Assistans ASM 2.1L. Our user friendly software PurityChrom® MCC and the required IT hardware are included too. Referring to the special needs of every separation, the SMB system can be freely configured (e.g. closed-loop, open-loop) and is upgradable (e.g. detector, flow meter). A maximum pressure up to 344 bar (eight columns). Depending on your application the columns can be heated or cooled to ensure the perfect and most constant separation conditions. It can be choosen between a stainless steel or a bioinert version.Read more
Software Solution to control and monitor AZURA multi column chromatography systems
PurityChrom® MCC is a special version of our purification software PurityChrom® and is optimized to be used with continuous chromatography Systems e.g. SMB systems. PurityChrom® provides a very user-friendly and clearly structured interface. The system visualization offers a graphical representation and allows easy handling even of complex flow processes. Furthermore each device which is displayed in the fluidic scheme can be manually controlled, giving the opportunity to optimize, change and adapt your conditions also during the run. The new PurityChrom MCC SMB parameter wizard helps you to generate new SMB methods and optimize your parameters while the process is running. With the integrated starting point calculator, you can easily generate you SMB method with the adsorption isotherms of your substances. The method editor allows you to freely design your own multi-column process. There is also a possibility to pause your method during a run. The hold function provides you with complete control over your chromatography process. Solvent visualization calculates the consumption of solvent for the current run and prevents your column from running dry. PurityChrom® allows flexible control of different devices from KNAUER and other manufacturers.
Build a custom solution based on your requirements. Just pick the components that match your method parameters.
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