Purification of epigallocatechin gallate and other related polyphenols from green tea by mass-triggered fractionation

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Application No.: VFD0177

Mass-triggered purification of epigallocatechin gallate from green tea extract

Epigallocatechin gallate is one of the major metabolites in green tea material and has shown positives effects on human health in several studies. This target molecule was isolated together with three other polyphenolic compounds in a short time with an AZURA® Preparative HPLC system using mass-triggered fractionation. The number of fractions was reduced to a minimum by this technique leading to a significant decrease in past analysis time showing that mass-directed purification is the ideal method in the isolation of natural products.

Technical Data

Application details

Method LC-MS
Mode RP
Substances epigallocatechin gallate, catechin
Key words Catechin, epigallcatechin gallate, polyphenols, green tea, mass-directed purification, LC-MS, mass spectroscopy, EGCG
CAS number 989-51-5

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