Epigallocatechin gallate is one of the major metabolites in green tea material and has shown positives effects on human health in several studies. This target molecule was isolated together with three other polyphenolic compounds in a short time with an AZURA® Preparative HPLC system using mass-triggered fractionation. The number of fractions was reduced to a minimum by this technique leading to a significant decrease in past analysis time showing that mass-directed purification is the ideal method in the isolation of natural products.
Analytical chromatogram of the crude green tea extract at 220 nm; step gradient separation 10 % acetonitrile until 26 min, then 15 % acetonitrile
UV chromatogram of a purification run for the crude green tea extract at 220 nm
SIM (single ion monitoring) chromatogram of a purification run for the target mass of m/z 457.4
Analytical chromatogram of the third fraction containing epigallocatechin gallate (m/z 457.4; [M-H]-); linear gradient separation 5 %-50 % acetonitrile
|Substances||epigallocatechin gallate, catechin|
|Key words||Catechin, epigallcatechin gallate, polyphenols, green tea, mass-directed purification, LC-MS, mass spectroscopy, EGCG|
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