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Purification of cannabidiol from CBD oil by preparative HPLC

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Application No.: VPH0074

Purification of cannabidiol from CBD oil by preparative HPLC


The demand of pure cannabidiol (CBD) is increasing and therefore methods are needed that allow purification of CBD in high purity from different kind of samples i.e. cannabis extract, paste or oil. Preparative HPLC is a well-established method for purification of target substances from natural substrates in high purities and was therefore applied for this task. In this application a preparative HPLC method was developed for purification of CBD from a commercial CBD rich oil. CBD was purified with high purity and recovery thus increasing the purity from 71.6% to 94.5%.

Technical Data

Application details

Method HPLC purification
Mode RP
Substances Cannabinoids
Key words Cannabis, hemp, purification, scale-up, preparative LC, CBD purification, CBDV, CBG, CBD, CBN, Δ9-THC, CBL, CBC
CAS number 24274-48-4, 2808-33-5, 13956-29-1, 521-35-7, 1972-08-3, 21366-63-2, 20675-51-8
Version Version 1 I 08/2020

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