Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and can be used as natural sugar substitutes, because they have a far higher sweetening power than normal sucrose or glucose. The sweetness is estimated to be about 400 times higher. This gradient method provides a fast determination of six steviol glycosides using reversed phase HPLC and UV detection. Furthermore, an automated matrix reduction is achieved by online SPE (solid phase extraction), speeding up sample preparation and guaranteeing a high sample throughput.
Measurement of washing process; blue area: matrix; 1) rebaudioside A, 2) stevioside, 3) rebaudioside C, 4) dulcoside A, 5) steviolbioside; 20 μL injection of Stevia extract; 0-7 min) measuring of SPE Washing Process, 7-15 min) measuring of HPLC
Measurement of sample only; 1) rebaudioside A, 2) stevioside, 3) rebaudioside C, 4) dulcoside A, 5) steviolbioside; 20 μL injection of Stevia extract
|Mode||RP, Online SPE|
|Key words||Stevia rebaudiana; steviol glycosides; rebaudioside A; stevioside; rebaudioside C; dulcoside A; rebaudioside B; steviolbioside; stevia sweetener; stevia; method transfer; Scale-Up; overload; HPLC; reversed phase; gradient; stevia sweetener; analytical; SPE; online SPE; automation; sample preparation; AZURA|
|CAS number||58543-16-1, 57817-89-7, 63550-99-2, 64432-06-0, 58543-17-2, 41093-60-1|
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