EN DE

Determination of six steviol glycosides using reversed phased HPLC and online SPE

Contact us
Application No.: VFD0174

Determination of six steviol glycosides using reversed phased HPLC and online SPE

Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and can be used as natural sugar substitutes, because they have a far higher sweetening power than normal sucrose or glucose. The sweetness is estimated to be about 400 times higher. This gradient method provides a fast determination of six steviol glycosides using reversed phase HPLC and UV detection. Furthermore, an automated matrix reduction is achieved by online SPE (solid phase extraction), speeding up sample preparation and guaranteeing a high sample throughput.

Technical Data

Application

Method HPLC

Separation conditions

Mode RP, Online SPE
Substances steviol glycosides
Key words Stevia rebaudiana; steviol glycosides; rebaudioside A; stevioside; rebaudioside C; dulcoside A; rebaudioside B; steviolbioside; stevia sweetener; stevia; method transfer; Scale-Up; overload; HPLC; reversed phase; gradient; stevia sweetener; analytical; SPE; online SPE; automation; sample preparation; AZURA
CAS number 58543-16-1, 57817-89-7, 63550-99-2, 64432-06-0, 58543-17-2, 41093-60-1

Documents and more information

Do you need more details or application support?

We are happy to help you

Follow us