Which UV-wavelength is appropriate for my HPLC application?

Certain buffers and organic solvents encumber the UV detection. A mobile phase with a UV cut-off below the detection wavelength will not interfere with the signal sensitivity. Examples for UV cut-off: water 185 nm, methanol 205 nm, acetonitrile 190 nm. A suitable wavelength for a mobile phase of acetonitrile/water has to be higher than 190 nm.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

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