What is RI detection?

With a refractometer, the so-called refractive index of a medium is determined by an optical measurement. The refractive index (n) is a quantity which relates the propagation velocity of light in a medium (cM) to the propagation velocity of light in vacuum or also the speed of light in vacuum (C0), which is defined as 1. Differential refractometry is a robust methodology that enables universal detection and has been accepted and established for many years.

Advantages: low price, easy handling, good linear behavior, universally applicable

Disadvantages: lower sensitivity than other universal detectors, not gradient-compatible


We separate molecules and unite people.

Based in Berlin, KNAUER is a medium-sized company that has been serving the sciences since 1962. We are a developer and manufacturer of scientific instruments of superior quality for liquid chromatography (LC), liquid dosing and other laboratory tasks. The product range includes systems, engineering solutions and components for:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-column Chromatography / SMB
High-pressure dosing solutions
Lipid nanoparticle (LNP) production and research

We are an established partner in science. Today and in the future.​​​​​​

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