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What is RI detection?

With a refractometer, the so-called refractive index of a medium is determined by an optical measurement. The refractive index (n) is a quantity which relates the propagation velocity of light in a medium (cM) to the propagation velocity of light in vacuum or also the speed of light in vacuum (C0), which is defined as 1. Differential refractometry is a robust methodology that enables universal detection and has been accepted and established for many years.

Advantages: low price, easy handling, good linear behavior, universally applicable

Disadvantages: lower sensitivity than other universal detectors, not gradient-compatible

 

We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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