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Purification of p-cumaric acid esterase by immobilized metal ion affinity chromatography

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Application No.: VBS0076

Purification of p-cumaric acid esterase by immobilized metal ion affinity chromatography

The p-cumaric acid esterase (p-CAE) has a wide range of applications and is therefore an enzyme of interest. p-CAE was produced by Pichia pastoris and purified by immobilized metal ion affinity chromatography (IMAC). Different IMAC systems were tested and compared. 35.4% of the total protein was identified as active p-CAE. A total activity loss of 21.4% was recorded. The p-CAE could be successfully purified.

Technical Data

Application

Method FPLC

Separation conditions

Mode Affinity
Substances p-cumaric acid esterase
Key words FPLC, Affinity, AZURA FPLC, IMAC, protein purifiaction
CAS number -

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