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Column choice based on Tanaka characterization - not all C18 columns are the same

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Application No.: VSP0014

Column choice based on Tanaka characterization - not all C18 columns are the same

Reversed Phase (RP) is with more than 90 % market share by far the most commonly used HPLC mode. The best known and most used surface modification is C18. Although the USP column code (L1) is the same for all C18 phases, there are a lot of differences which must be considered when choosing the right column. C18 always is a good choice for an initial try but one must bear in mind that not all C18 phases have the same separation characteristics. To differentiate between such phases Tanaka plots are extremely useful.

Technical Data

Application

Method HPLC

Separation conditions

Mode RP
Substances uracil, butyl benzene, o-terphenylen, pentyl benzene, triphenylen
Key words Tanaka, reversed phase, column characterization, C18, Tanaka plot, hydrophobic retention, shape selectivity
CAS number 66-22-8, 104-51-8, 84-15-1, 538-68-1, 217-59-4

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