In many cases protein purification requires two or even more steps to achieve sufficient purity. The transition from one step to another can be very time consuming and requests manual interaction. These limitations illustrate the need for FPLC systems enabling automated two step purification. This application highlights the possibilityof combining two subsequent chromatography protocols. This was achieved using the KNAUER Lab standard Multi Method FPLC system adapted for automatic two step purification. An exemplary separation of model proteins by cation exchange chromatography followed by desalting is shown. This approach can easily be adapted to various protein purification protocols.
Chromatogram of the cation exchange method.
|Method||Two step purification|
|Substances||Cytochrome C, Ribonuklease A|
|Key words||FPLC, IEC, protein purification, Cytochrome C, Ribonuklease A, AZURA® Bio Lab system|
|CAS number||Cytochrome C: CAS 9007-43-6; Ribonuklease A: CAS 9001-99-4|
|Version||Version 1 / 12/2020|
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