This application note describes an automated purification of mouse immunoglobulin antibodies (IgG) from cell culture. The cell culture was applied with a feeding pump on a proteinA affinity column to capture and purify the antibodies. These were kept in the system and in a second step applied on a gel filtration column for buffer exchange. The obtained antibody was now dissolved in the desired storage and/or working buffer. This application note highlights the possibility of automated purification with the AZURA BIO LC system without manual interaction during purification process.
|Substances||Immunoglobulin G 1 (IgG1) from mouse|
|Key words||IgG1, cell culture, antibodies, FPLC, proteinA – affinity, SEC – buffer exchange|
|CAS number||not available|
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