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Automated two - step purification of mouse antibody IgG1 with AZURA Bio LC Lab system

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Automated two - step purification of mouse antibody IgG1 with AZURA Bio LC Lab system
Application No.: VBS0063

This application note describes an automated purification of mouse immunoglobulin antibodies (IgG) from cell culture. The cell culture was applied with a feeding pump on a proteinA affinity column to capture and purify the antibodies. These were kept in the system and in a second step applied on a gel filtration column for buffer exchange. The obtained antibody was now dissolved in the desired storage and/or working buffer. This application note highlights the possibility of automated purification with the AZURA BIO LC system without manual interaction during purification process.

 

Technical Data

Application

Method FPLC

Separation conditions

Mode Affinity, SEC
Substances Immunoglobulin G 1 (IgG1) from mouse
Key words IgG1, cell culture, antibodies, FPLC, proteinA – affinity, SEC – buffer exchange
CAS number not available

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