Eurospher II is KNAUER´s workhorse for an even wider range of application areas in classical HPLC, fast HPLC Plus and ultra-fast UHPLC. Based on an ultra pure spherical silica gel, Eurospher II is a high performance column material for analytical, semipreparative and process-scale applications.

Eurospher II features very narrow particle and pore size distributions, as well as outstanding mechanical stability. Eurospher II silica gel is perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks.

  • Physical properties
  • Endcapping
  • Modifications
  • Selectivities
  • Physical properties of Eurospher II

    Eurospher II offers outstanding mechanical and chemical stability. With physical properties very similar to those of Kromasil 100, Eurospher II columns can be used to replace Kromasil® columns, providing excellent peak symmetry for acids, bases, and neutrals. Compared to Kromasil® 100, Eurospher II has

    • nearly the same particle shape
    • a lower metal impurity specification
    • higher mechanical stability
    • comparable selectivity in RP mode (Eurospher II C18 H vs. Kromasil® 100 C18)

    Silica gel

    ultra pure, > 99.99 %

    Metal content

    < 10 ppm

    Particle size

    2 μm, 3 μm, 5 μm, 10 μm (15 μm, 20 – 45 μm upon request)

    Particle form


    Pore size

    100 Å

    Specific surface

    320 ± 20 m2/g

    Pore volume

    0.8 ml/g


    430 g/l

  • Endcapping styles of Eurospher II materials

    With several different levels of endcapping, Eurospher II offers a wide variety of surface modification types for analytical as well as preparative columns. Our long experience and knowledge in producing HPLC columns ensures the highest reproducibility.

    Four different Eurospher II C18 types are now available. Eurospher II reversed phase columns are up to the challenge by delivering outstanding selectivity of positional isomers, steric isomers, and polar compounds.

    Whether your application requires high pH or low pH conditions, Eurospher II C18 H, and C18 P columns perform equally well.

  • Modifications of Eurospher II

    With the wide range of different surface modifications available, all application fields in reversed phase and normal phase modes are covered. Every Eurospher II modification offers high chemical stability and loading capacity thanks to mono- and multi-functional silanes.

    Modification USP code Carbon % pH range
    C18 P L1 20% (~ 99% endcapping) 1 - 12
    C18 H L1 17% (> 99% endcapping) 1 - 12
    C18 L1 16% (~ 50% endcapping) 2 - 8
    C18 A L1 10% (~ 50% hydrophilic endc.) 2 - 8
    Phenyl L11 12% (~ 50% endcapping) 2 - 8
    C8 L7 10% (~ 50% endcapping) 2 - 8
    C8 A L7 8% (> 50% endcapping) 2 - 8
    C4 L26 7% (~ 50% endcapping) 2 - 8
    NH2 L8 4% (no endcapping) 2 - 8
    CN L10 7% (no endcapping) 2 - 8
    HILIC - 7% 2 - 8.5
    Diol L20 5% (no endcapping) 2 - 8
    Si L3 0% (no endcapping) 2 - 8

  • Selectivities of Eurospher II

    Selectivity plots for phases are based on Tanaka tests.   

    Selectivity plots for phases are based on Tanaka tests: HR: hydrophobic retention | HS: hydrophobic selectivity | SS: steric selectivity | HBC: hydrogen bonding capacity | IEX pH 7.6: ion exchange capacity at pH 7.6 | IEX pH 2.7: ion exchange capacity at pH 2.7                                                                 

  • Applications and separation mechanisms of Eurospher II columns
  • Eurospher II application areas
  • Applications and separation mechanisms of Eurospher II columns

    Modification Application areas Separation mechanism
    C18 P alternative selectivity to standard C18; stationary phase in Eurospher II C18 family with the highest carbon load; fully endcapped; excellent shape selectivity and pH stability hydrophobic and steric interaction
    C18 H recommended alternative for Kromasil 100 C18 columns; for acidic, basic and neutral analytes in reversed phase mode with extended pH range hydrophobic interaction
    C18 for acidic, basic and neutral analytes in reversed phase mode (sulphonamides; anabolic steroids; anti-psychotics; beta blocker; Sudan dyes; phenols, preservatives etc.) hydrophobic interaction
    C18 A polar endcapped C18 phase for alternative selectivity; 100 % aqueous eluents for analysis of very polar compounds, basic pharmaceutical ingredients, water soluble vitamins, catecholamines as well as organic acids hydrophobic and polar interaction
    C8 similar selectivity to C18 phase but less retention due to the lower hydrophobicity; useful for determination of water soluble vitamins, steroids, catecholamines, sedatives etc. reduced hydrophobic interaction comparing to C18 phase
    C8 A alternative selectivity to C8 with stronger interactions for polar compounds; 100% aqueous applications with very polar compounds. hydrophobic, hydrophilic and polar interaction
    C4 universal packing material for different application areas; can also be used in HIC mode (Hydrophobic Interaction Chromatography) hydrophobic and hydrophilic interaction
    Phenyl alternative selectivity for aromatic and mode rately pi-pi interaction polar analytes or mixtures with varying polarity and aromaticity pi-pi interaction with aromatics
    CN for a wide range of application in normal pase mode as well as reversed phase mode (steroids, carbohydrates polar compounds) hydrophobic and hydro philic interaction
    (ammonium-sulfonic acid)
    especially suited for the separation of hydrophilic, polar and ionic analytes which are only poorly retained on reversed phase columns; behavior is the other way round on Eurospher II HILIC compared to RP which makes it an ideal tool to enhance chromatographic separations for these molecules hydrophilic and weak electrostatic interaction
    Diol alternative to the silica packing with shorter equilibration time and comparable selectivity hydrophilic interaction
    NH2 most flexible phase in the Eurospher II family; can be used in three modes: normal phase, reversed phase and ion chromatography mode (weak anion exchanger); different selectivity to the silica packing; in reversed phase mode mainly used for analysis of carbohydrates hydrophilic and ionic interaction
    Si wide range of different applications, i.e. SEC (size exclusion chromatography) but also for normal phase HPLC; good choice for analytical and preparative purposes to separate polar compounds hydrophilic interaction

  • Eurospher II application areas

    The choice of the appropriate column for a particular application can be a daunting task. With a range of bonded phases offering different selectivity, the Eurospher II family includes columns to meet most separation needs. The table below will help you to choose the best Eurospher II column for a particular application.

Highly reproducible columns

Eurospher II offers an outstanding batch-to-batch reproducibility

Applications with Eurospher II Columns

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