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Separation of Pyrimidine and Purine Bases in HILIC mode with AZURA Compact

Application No.: VBS0035J

Separation of Pyrimidine and Purine Bases in HILIC mode with AZURA Compact

The separation of compounds chosen among purine and pyrimidine bases was studied by using hydrophilic interaction chromatography (HILIC). The retention of the analytes was studied by varying ACN%, ammonium formate concentration, pH, and column temperature. The results obtained confirmed the elution order of nucleobases and nucleosides on their hydrophobicity.

Technical Data

Application

Method HPLC

Separation conditions

Mode HILIC
Substances 3,5- Di-tert-butyltoluene, Adenine, Cytosine, Uracil
Key words HILIC, AZURA Compact, Eurospher II HILIC, Purine, Pyrimidine
CAS number 15181-11-1, 66-22-8, 73-24-5, 71-30-7

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