Two-step CBG purification – combined matrix removal and peak recycling

Application No.: VPH0077

Two-step CBG purification – combined matrix removal and peak recycling

Recycling chromatography is a powerful technique that allows the separation of two closely eluting peaks by increasing the column bed length. The technique allows the eluted substances to be recycled after passing through the column. Thereby, critical peak pairs that elute together can be separated further with each cycle. A previously presented recycling system was upgraded by adding, two additional valves. This optimization enables 1) peak parking for removal of impurities from the target compounds and 2) re-injection of the peak pair back into the system for peak recycling. The versatility of the recycling system is greatly enhanced by this upgrade. In this application, the optimized recycling system was used to remove matrix components from CBG-rich cannabis extract, allowing subsequent separation of CBG (cannabigerol) and CBD (cannabidiol) for purification of CBG. By combining two separation techniques in one system, CBG was purified to a high degree of purity, increasing from 81 % to 100 %.

Technical Data

Application details

Method Preparative HPLC
Mode RP
Substances Cannabigerol (CBG), Cannabidiol (CBD)
Key words HPLC, Semi-preparative chromatography, preparative chromatography, RP, peak recycling, peak parking, matrix removal, cannabinoids, Cannabigerol (CBG), Cannabidiol (CBD)
CAS number 25654-31-3, 13956-29-1
Version Version 1/ 11/2023

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