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Systematic HPLC method development and robustness evaluation of 13 carbonyl DNPH derivatives using DryLab

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Systematic HPLC method development and robustness evaluation of 13 carbonyl DNPH derivatives using DryLab
Application No.: VEV0078

In the monitoring of industrial air, the determination of carbonyl (aldehyde and ketone) emissions is crucial to prevent respiratory, pulmonological, autoimmune diseases, and cancer. According to the analytical method described in the DIN ISO 16000-3 standard [1], the carbonyls must be converted to their corresponding hydrazones with 2,4-dinitrophenylhydrazin (DNPH) in order to be detected via UV detector and analyzed by reversed phase HPLC. Here, the DryLab® software was used for method optimization to separate of 13 carbonyl derivatives in a standard mixture with the AZURA® HPLC system and the DNPH-column.

Technical Data

Application details

Method HPLC
Mode RP
Substances Acetaldehyde-DNPH, Acetone-DNPH, Acroleine-DNPH, Benzaldehyde-DNPH, 2-Butanone-DNPH, n-Butyraldehyd-DNPH, Crotonaldehyde-DNPH, Formaldehyde-DNPH, Hexaldehyde-DNPH, Methacroleine-DNPH, Propio
Key words Aldehyde- and Ketone-DNPH, DryLab, method optimization, UHPLC
CAS number 1019-57-, 1567-89-1, 888-54-0, 1157-84-2, 958-60-1, 1527-98-6,1527-96-4, 1081-15-8, 1527-97-5, 5077-73-6, 725-00-8, 2880-5-9, 2057-84-3

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