Small-scale SEC with AZURA® compact FPLC

Application No.: VBS0080

Small-scale SEC with AZURA® compact FPLC

Size-exclusion chromatography (SEC) separates molecules depending on differences in their size. SEC is frequently used as a polishing step during protein purification and can also be used for the analysis of protein homogeneity.

In recent years the use of small-scale SEC for the analysis of low sample volumes or for the purification of small sample amounts has become more important; especially due to the development of suitable columns.

Here, we performed a proof-of-concept study to evaluate the performance of the AZURA® compact SEC system for the use of small-scale SEC applications and describe the system configuration for this application.

Technical Data

Application details

Method FPLC
Mode SEC
Substances Thyroglobulin, gamma-globulin, ovalbumin, myoglobin, Vitamin B12
Key words FPLC, SEC, gel filtration, size exclusion chromatography, protein purification, protein separation, antibody purification
CAS number -
Version Version 1/12/2021

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