When working with preparative liquid chromatography (LC) pumps such as the AZURA P 2.1L some degree of pressure pulsation is to be expected, due to the operating principles of piston pumps. Although the P 2.1L is equipped with active pulsation compensation, residual pulsation cannot be prevented. Residual pulsation is generally not an issue but for certain applications, such as precise dosing or when working with sensitive columns, it can be problematic. Integration of the KNAUER PEEK Pulse Damper into the LC system can mitigate the pump’s pressure pulsation. In the conducted experiments the effect of the pulse damper was most visible with high flow rates up to 100 ml/min.
Integration of a pulse damper in a typical preparative LC-System
Effect of pulse damper on pressure amplitude; red – without pulse damper; blue with pulse damper; flow rate 80 ml/min
Average pulsation dampening at flow rates of 10 to 100 ml/min ± relative standard deviation (RSD)
|Key words||pulse damper, preparative HPLC|
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