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Oh so sweet - Quantification of steviol glycosides in Stevia samples with RP-HPLC

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Application No.: VFD0168

Oh so sweet - Quantification of steviol glycosides in Stevia samples with RP-HPLC

Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and can be used as natural sugar substitutes. This method provides a fast determination of six steviol glycosides using the AZURA® HPLC Plus System. With a fast separation under 10 min and an optimized gradient, the developed method is suitable for a fast quality control of stevia products. Furthermore, the robustness for this method was validated using DryLab simulation software.

Technical Data

Application details

Method HPLC
Mode RP
Substances Rebaudioside A, stevioside, rebaudioside B, rebaudioside C, dulcoside a, steviolbioside
Key words Stevia rebaudiana, steviol glycosides, rebaudioside A, stevioside, rebaudioside C, dulcoside A, rebaudioside B, steviolbioside, stevia sweetener, Stevia, SPE, sugar substitute
CAS number 58543-16-1, 57817-89-7, 63550-99-2, 64432-06-0, 58543-17-2, 41093-60-1

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