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How to prevent the error message NO FREEZE?

Cause Solution

The freeze temperature set in the temperature menu (COOL) is too high for the sample.

Lower the freeze temperature gradually by steps of 0.5 °C. Please be aware that after the settings have been changed, a new calibration is required.

The cooling limit in the temperature menu is too high.

Lower the cooling limit in the temperature menu gradually by steps of 0.5 °C to accelerate the cooling process. Please be aware that after the settings have been changed, a new calibration is required.

The volume of the sample solution is too high, which prohibits freezing of the sample.

Use an appropriate sample volume as it is recommended in the KNAUER user manual.

The used plastic vial is not suitable for the measurement.

Use the plastic vials that are recommended by KNAUER.

 

 

 

 

 

 

 

 

We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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