How to avoid air entering the FPLC system?

There are some simple rules, which you can follow to prevent air entering the system. Never place bottles below the pump. Make sure that your buffer temperature is also your working temperature. Degas your buffers. Make sure that all tubing connections are tightened. Check the pump inlets if air is drawn in there. 

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Analytical HPLC / UHPLC
Preparative HPLC
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