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How can reversed phase HPLC columns be cleaned and regenerated?

This is our recommended regeneration procedure for RP packings C18, C8, C4, C1, C30, CN and Phenyl stationary phases:

Flush the column with 20 column volumes of water
Flush the column with 20 column volumes of acetonitrile
Flush the column with 5 column volumes of isopropanol
Flush the column with 20 column volumes of heptane
Flush the column with 5 column volumes of isopropanol
Flush the column with 20 column volumes of acetonitrile

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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