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How can I replace or install a precolumn on an analytical KNAUER column with 3 - 4.6 mm ID?

How to remove and how to reinstall an integrated precolumn to a KNAUER HPLC column:

 

Tools Required:

Open end wrenches (1/4’’ and 13 mm)

Replacement Precolumn Cartridges:

Depend on the stationary phase in the analytical column. If you are not sure just contact KNAUER to choose the best fitting precolumn.

Vertex III Precolumn screw cap A0028-2:

For Vertex III column without integrated precolumn, when a precolumn is installed. Not needed when a precolumn cartridge is replaced at a Vertex III column with integrated precolumn.

Procedure:

  1. Wait for the column to depressurize before replacing or installing a cartridge.
  2. Remove the Column screw cap using the 1/4’’ and 13 mm wrenches at the spanner flat at the column and on the screw cap. Don´t be afraid, column material cannot get out. It is fixed with sieves and seals.
  3. If a precolumn is first installed: Use only the endfitting from the Vertex III column. Use the connector and precolumn screw cap from A0028-2. If a precolumn cartridge is replaced: Use the endfitting, the connector and the precolumn screw cap from the Vertex III column. Special case: If you are using a Vertex Plus column (delivered until 02/2019) and are now installing a Vertex III precolumn, please refer to the supplement V7623 delivered with the new precolumns.
  4. Assemble the column and your chosen precolumn cartridge as indicated below. Use the 1/4’’ and 13 mm wrenches to fasten the precolumn screw cap.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

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