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Group separation with Sepapure Desalting on AZURA® Bio purification system

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Application No.: VBS0075

Group separation with Sepapure Desalting on AZURA® Bio purification system

Size exclusion chromatography (SEC) is a popular FPLC techniques used for protein purification. Molecules are separated according to their size. Depending on the aim of the purification high resolution fractionation or group separation is used. This application describes group separation with Sepapure Desalting and shows examples for the separation of proteins from dyes and for protein desalting.

Technical Data

Application

Method FPLC

Separation conditions

Mode SEC
Substances Bovine Serum Albumin (BSA), 5-Carboxyfluorescein (5-FAM)
Key words SEC, Desalting, Buffer exchange, group seperation, FPLC, Sepapure Desalting, AZURA FPLC
CAS number 9048-46-8, 76823-03-5

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