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Fast and robust purification of antibodies from human serum with a new monolithic protein A column

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Fast and robust purification of antibodies from human serum with a new monolithic protein A column
Application No.: VBS0068

The use of antibodies is becoming more and more important. Therewith the need for fast and robust methods for their purification and columns with a long-life time is growing. In this application we describe a fast and robust protein A affinity chromatography method with a new monolithic column. Antibodies were successfully purified in less than 1.5 minutes with a flowrate of 5mL/min highlighting the advantages of this column and method.

Technical Data

Application

Method FPLC

Separation conditions

Mode Affinity
Substances human serum, antibodies
Key words Human Serum, Antibody, Protein A, Affiniy chromatography, Monolithic column
CAS number -

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