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Evaluation of a pulse damper for FPLC systems with a P 6.1L pump

Application No.: VTN0020

Evaluation of a pulse damper for FPLC systems with a P 6.1L pump

Pressure fluctuations caused by pump pulsation can present a challenge in FPLC, especially when working with pressure-sensitive columns. Here we analyzed the performance of the KNAUER PEEK pulse damper in combination with the AZURA® P 6.1L pump. The pulse damper was able to reduce pressure pulsation by up to 67% at small flow rates. The best working conditions for the pulse damper were determined at flow rates below 1ml/min in combination with a minimum back pressure of approximately 1.5 bar. At flow rates above 1ml/min, the dampening efficiency decreases significantly

Technical Data

Application details

Method FPLC
Mode SEC
Substances -
Key words FPLC, SEC, pulse dsamper, P6.1L, biocomaptible
CAS number -
Version Version 1 / 10/2021

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