Cyclodextrins (CD) are macrocyclic compounds composed of five or more glycopyranosides. These ring structures can function as micro-capsules. An automated column screening was applied for method development to find best separation conditions of a CD mixture. Finally, an optimized method for CD purification was established.
Comparison of separation profiles from CD mixture and CD standards on C18 (dark blue) and C18H (red) columns (relevant time span of separation shown). CD mixture (30 mg/mL), CD8 (1mg/mL), CD 12,9,8 mixture (3mg/mL), CD10, 11 mixture (2 mg/mL); Y, Z uniden
Chromatograms of CD mixture mass overload studies; grey – 25 mg/ mL, green – 50 mg/mL, red – 75 mg/mL, dark blue – 100 mg/mL; C18 H 250 x 4 mm; 5 μm; 0.8 mL/min; 3 % methanol; 25°C; 50 μL
Chromatograms of CDmix volume overload studies. green – 50 μL, red – 75 μL, dark blue – 100 μL, light blue – 200 μL; C18H 250 x 4 mm; 5 μm; 0.8 mL/min; 3 % ,methanol; 25°C; 50 mg/mL
|Substances||Cyclodextrine (CD), CD8, CD9, CD10, CD11, CD12|
|Key words||Cyclodextrine mixture, preparative LC, RP, pharma, synthesis|
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