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Silphium perfoliatum

Characterization of fructans in fermentation products with GPC/SEC

Application No.: VBS0079

Characterization of fructans in fermentation products with GPC/SEC

 

Recent efforts to improve the efficiency of biorefineries aim to take advantage of otherwise discarded side products. In this application, a sample of biotechnological origin from cup plant (Silphium perfoliatum) was characterized in regards of the distribution of fructan polymers and other sugar contents. This was achieved using size exclusion chromatography (SEC) in combination with refractive index detection (RID). Next, the method was scaled up to a semi-preparative dimension in order to separate oligomers from monomers.

Technical Data

Application details

Method GPC/SEC
Mode GPC/SEC
Substances Fructan polymers
Key words Biotechnology, purification, size exclusion chromatography, refractive index detection, cup plant stems, fructans, oligomers, polymers, monomers, gel permeation chromatography
Version Version 1 / 03/2021

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