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Can I run a reversed phase column with 100 % aqueous mobile phase?

It is definitely not recommended to run a common C18 column with pure aqueous mobile phase. Because of their length the linked chains have hydrophobic properties and might collapse if used with 100 % water.

As an alternative special columns with a hydrophilic/polar endcapping, like KNAUER Eurospher II C18 A, can be used.

 

 

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