EN DE

Automated two-step purification of 6xHis-tagged GFP with AZURA Bio LC

Contact us

Automated two-step purification of 6xHis-tagged GFP with AZURA Bio LC
Application No.: VBS0067

Affinity chromatography by His-Tag is one of the most widespread purification techniques for recombinant proteins. In many cases it requires an additional cleaning/polishing step. This application highlights the possibility of combining two subsequent chromatography protocols without manual interaction using the AZURA Bio LC system.

Technical Data

Application

Method FPLC

Separation conditions

Mode AC, SEC
Substances Green fluorescence protein (GFP) with 6xHis - Tag
Key words GFP, His-Tag, IMAC, FPLC, affinity, SEC, buffer exchange
CAS number -

Downloads and more information

Do you need more details or application support?

We are happy to help you

Follow us