Are there limitations relating to mobile phase properties?

Do not use highly basic or acidic solvents unless your HPLC system and applied column are able to withstand these properties. Seals and other wetted parts can be damaged by extreme pH conditions. Highly aqueous mobile phase can only be used with certain columns. A high water content in the mobile phase can promote bacterial growth. To prevent this, the system/column needs to be flushed with organic solvent in regular intervals.

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