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Are there limitations relating to mobile phase properties?

Do not use highly basic or acidic solvents unless your HPLC system and applied column are able to withstand these properties. Seals and other wetted parts can be damaged by extreme pH conditions. Highly aqueous mobile phase can only be used with certain columns. A high water content in the mobile phase can promote bacterial growth. To prevent this, the system/column needs to be flushed with organic solvent in regular intervals.

We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB
Osmometry

We are an established partner in science. Today and in the future.​​​​​​

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