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What is the typical loadability and flow rate of preparative HPLC columns?

The mass and volume loadabilities always depend on the specific sample and application as well as on the stationary phase filled in the HPLC column. The following chart only gives a first clue and can be higher or lower than the given numbers. Scale-up factor (SF) calculation: SF = ID²(preparative)/ID²(analytical)

  • Example: Scale-up from 250 x 4 mm ID column to 250 x 20 mm ID column: SF = ID²(preparative)/ID²(analytical) = 20²/4² = 400/16 = 25

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