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Separation of proteins with cation exchange chromatography on Sepapure SP and CM

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Separation of proteins with cation exchange chromatography on Sepapure SP and CM
Application No.: VBS0072

Ion exchange chromatography is one of the most widely used FPLC techniques for protein separation and purification. Depending on the charge of the sample and the resin cation or anion exchange chromatography is used. This
application describes an easy separation of model proteins and explains how cation exchange chromatography works.

Technical Data

Application

Method FPLC

Separation conditions

Mode IEC
Substances Cytochrome C, Lysozyme, Ribonuclease A
Key words FPLC, IEC, CIEC, AZURA FPLC, Sepapure SP, Sepapure CM
CAS number 9007-43-6,12650-88-3, 9001-99-4

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