Commonly the analysis of highly polar compounds is realized with ion exchange (IEX) or ion chromatography (IC) and so users are often limited to particular methods(1). These methods usually go along with the use of suppressors or other additional equipment to enable the detection by conductivity measurement. Using a special HILIC mixed-mode column in combination with evaporative light scattering detection (ELSD) allows to apply a simple isocratic method with a standard LC system configuration.
Chromatogram of KH2PO4, (1) H2PO4 -, (2) K+
Chromatogram of NaClO3, (1) ClO3 -, (2) Na+
Chromatogram of NaCl, (1) Cl-, (2) Na+
Chromatogram of KI, (1) K+, (2) I
Chromatogram of LiBr, (1) Br-, (2) Li+
Chromatogram of mixed standard, (1) H2PO4 -, (2) Cl-, (3) Br-, (4) ClO3 -, (5) Na+, (6) Li+, (7) K+, (8) I-
Sample results for tap water, red – Na+, grey – Cl-
Overlay of tap water samples, (1) Cl-, (2) Na+
Overlay of mineral water samples, (1) Cl-, (2) Na+, (3) K+
| Method | HPLC |
|---|---|
| Mode | HILIC |
| Substances | lithium bromide, sodium chloride, potassium dihydrogenphosphate, sodium chlorate, potassium iodide |
| Key words | HILIC, IC, IEX, mixed-mode, inorganic ions, monovalent ions, ELSD, evaporative light scattering detection, anions, kations |
| CAS number | 7550-35-8, 7647-14-5, 7778-77-0, 7775-09-9, 7681-11-0 |
| Version | Version 1/ 10/2021 |
AZURA® Pump P 6.1L (HPG), with 10 ml pump head (stainless steel), degasser and mixer (400 µl)
Article No.: APH35EA
Show product
AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 862 bar
Article No.: AAA50AA
Show product
Column thermostat AZURA CT 2.1 for constant temperatures and reproducible results
Article No.: ATC00
Show product
Ultra high sensitive light scattering detector ELSD SEDEX 100LT for univ. detection 200 µl/min - 2 ml/min 100 Hz including accessories, SAGA
Article No.: A0754-6
Show product
OpenLAB® CDS EZChrom Edition workstation for one system with SMA and 4x System Suitability
Article No.: A2600-1
Show product
OpenLAB® CDS EZChrom Edition drivers for 80LT, 85LT, 90LT, 100LT and LC from Sedere
Article No.: A2618-01
Show productWe are happy to help you
