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How to recondition the HPLC column performance?

As first you can follow the cleaning and regeneration procedure on the back of your column certificate and maybe change the filters, sieves and precolumn. Next step should be re-running the standard test conditions as shown in the enclosed certificate and check the retention time, asymmetry and the theoretical plates. If these treatments have no effect on your separation capacity, the column has to be replaced.

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Analytical HPLC / UHPLC
Preparative HPLC
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