How to recondition the HPLC column performance?

As first you can follow the cleaning and regeneration procedure on the back of your column certificate and maybe change the filters, sieves and precolumn. Next step should be re-running the standard test conditions as shown in the enclosed certificate and check the retention time, asymmetry and the theoretical plates. If these treatments have no effect on your separation capacity, the column has to be replaced.

We separate molecules and unite people.

Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

We are an established partner in science. Today and in the future.​​​​​​

Do you need more information?

Please contact us!

Follow us