How to deal with fronting peaks?

The problem with fronting peaks can occur due to following reasons:


a) Interference in sample. Solution: Check column performance with standards. b) Shoulder or gradual baseline rise before a main peak may be another sample component. Solution: Increase efficiency or change selectivity of system to improve resolution. Try another column type if necessary. c) Column overloaded. Solution: Inject smaller volume or dilutions (e.g. 1:10 or 1:100) of sample. d) Sample solvent incompatible with mobile phase. Solution: Adjust solvent: Whenever possible, inject samples solved in mobile phase. Flush polar bonded phase column with 200 ml HPLC grade ethyl acetate, then with intermediate polarity solvent prior analyses.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

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