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How can polymer based columns be cleaned and regenerated?

Specific regeneration methods must be used for different types of polymer columns. Appropriate regeneration methods for specificpolymer based columnsare described below. This is our recommended regeneration procedure of polymer columns:

  • Eurokat H, CarbEx H-Form, Hamilton HC-75 Hydrogen Form
    Rinse the column for 4 to 6 hours with 0.05 N sulphuric acid at 60 °C, using aflow rate of 0.2 ml/min
  • Eurokat Ca, CarbEx Ca-Form, Hamilton HC-75 Calcium Form,Hamilton HC-40 Calcium Form
    Rinse the column for 4 to 6 hours with a 0.25 M calcium nitrate solution at 60 °C, using a flow rate of 0.2 ml/min
  • Eurokat Pb, CarbEx Pb-Form, Hamilton HC-75 Lead Form
    Rinse the column for 4 to 6 hours with a 0.25 M lead nitrate solution at 60 °C, using a flow rate of 0.2 ml/min
  • Hamilton PRP-1, PRP-3, PRP-Infinity
    Rinse the column with 20 column volumes of water
    Run a linear gradient from 100% water to 100% acetonitrile
    Repeat the procedure 3 times
  • Hamilton PRP X-100
    Rinse the column with 20column volumes of water
    Rinse the column with 20 column volumes of methanol with 1% 6 N nitric acid
  • Hamilton PRP X-200, PRP X-300
    Rinse the column with 20 column volumes of water
    Inject 100 μl of 1 N nitric acid, 5 times consecutively
  • Hamilton RCX-10
    Rinse the column with 20 column volumes of a 0.1 N sodium hydroxide solution
  • Hamilton RCX-30
    Rinse the column with 60 column volumes of a 0.1 N sodium hydroxide solution

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
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Multi-Column Chromatography / SMB
Osmometry

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