How can I sustain the capability of my HPLC column?

There are four major influences that have to be considered: eluent, sample, pH value and temperature. To prevent column plugging, it is reasonable to filter the samples and the eluent before usage. Furthermore the compatibility of the mobile phase is an important factor. Standard RP phases are stable within a pH range 2-8. Under certain conditions (presence of buffers) the stationary phases are even chemically stable until pH 12. When using buffered mobile phases it is necessary to flush the silica-based column with pure solvents to avoid precipitation of buffer salt. After usage all columns have to be plugged to avoid the stationary phase to dry out. Continuous operation at high temperatures will also decrease the capabilitiy. The recommended temperature limit for silica-based columns should be 60°C.

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Based in Berlin, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:

Analytical HPLC / UHPLC
Preparative HPLC
Fast protein liquid chromatography (FPLC)
Multi-Column Chromatography / SMB

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