Amino acid analysis is a considerable application applied in research, clinical facilities and industrial processes. The application described in this study uses o-Phtalaldehyde (OPA) as pre-column derivatizing reagent. OPA is a well-known and reliable derivatization reagent for amino acid analysis. Its significantly lower price compared to other derivatization reagents like 6- aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) makes this reagent very attractive for the use in routine analyses.
1. Asp; 2. Glu; 3. Ser; 4. Gly; 5. Thr; 6. His; 7. Ala; 8. Arg; 9. Tyr; 10. Val; 11. Cys; 12. Met; 13. Ile; 14. Phe; 15. Leu; 16. Lys
| Method | HPLC |
|---|
| Mode | RP |
|---|---|
| Substances | Aspartic acid, Glutamic acid, Serine, Glutamine, Histidine, Glycine, Threonine, Arginine, Alanine, Tyrosine, Methionine, Valine, Phenylalanine, Isoleucine, Leucine, Lysine |
| Key words | Amino acids, Precolumn derivatization, OPA, protein |
| CAS number | 56-84-8, 56-86-0, 56-45-1, 56-85-9, 71-00-1, 56-40-6, 72-19-5, 56-41-7, 60-18-4, 63-68-3, 72-18-4, 63-91-2, 73-23-5, 61-90-5, 56-87-1 |
Article No.: 25CF185PSG
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