Determination of Amino acids with OPA

Application No.: VPH0010J

Determination of Amino acids with OPA

Amino acid analysis is a considerable application applied in research, clinical facilities and industrial processes. The application described in this study uses o-Phtalaldehyde (OPA) as pre-column derivatizing reagent. OPA is a well-known and reliable derivatization reagent for amino acid analysis. Its significantly lower price compared to other derivatization reagents like 6- aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) makes this reagent very attractive for the use in routine analyses.

Technical Data


Method HPLC

Separation conditions

Mode RP
Substances Aspartic acid, Glutamic acid, Serine, Glutamine, Histidine, Glycine, Threonine, Arginine, Alanine, Tyrosine, Methionine, Valine, Phenylalanine, Isoleucine, Leucine, Lysine
Key words Amino acids, Precolumn derivatization, OPA, protein
CAS number 56-84-8, 56-86-0, 56-45-1, 56-85-9, 71-00-1, 56-40-6, 72-19-5, 56-41-7, 60-18-4, 63-68-3, 72-18-4, 63-91-2, 73-23-5, 61-90-5, 56-87-1

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