Since the change in German law in 2017 (§1 Abs. 1, BtMG)  the need for reliable and robust HPLC methods for quality control has drastically increased. Six common cannabinoids of high medicinal interest cannabidiol (CBD), cannabidiolic acid (CBDA), cannabinol (CBN), Δ8-tetrahydrocannabinol (Δ8-THC), Δ9-tetrahydrocannabinol (Δ9-THC), and Δ9-tetrahydrocannabinolic acid (Δ9-THCA) were quantified using
the AZURA® HPLC plus system according to the monography of German Pharmacopeia . Three different reversed phase columns were screened for robustness for the method verified in the subsequent step. Method verification took place using authorized medicinal cannabis flower samples available on the market. The substances were analysed with the help of chemical reference standards.
Representation of the robust space using the tG-T-pH (30/90 min, 30/50°C, pH 1.6/2.2/2.8) with the columns C18 (left), C18H (center) and C18P (right), adjusted robust space level 1.61.
Chromatogram of standard mix of 10 μg/mL, R=1.5 between Δ8- THC and Δ9-THC; blue - 225 nm, red - 306 nm, 1 - CBDA, 2 - CBD, 3 - CBN, 4 - Δ9-THC, 5 - Δ8-THC, 6 - Δ9-THCA.
|Sample||CBD, CBDA, CBN. THC, THCA|
|Separation column||Eurospher II C18P 4,6 x 150 mm, 3μm, 100 A|
|Key words||bediol, DAB, CBN, THC, DABA, THCA, cannabis, german pharmacopeia, cannabinoids, cannabis flowers, cannabis sativa L.|
|CAS number||13956-29-1, 1244-58-2, 521-35-7, 5957-75-5, 1972-08-3, 23978-85-0, 75-05-8, 64-17-5, 7664-38-2|
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