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Analyzing cannabis flowers according to the German Pharmacopeia

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Application No.: VPH0069

Analyzing cannabis flowers according to the German Pharmacopeia

Since the change in German law in 2017 (§1 Abs. 1, BtMG) [1] the need for reliable and robust HPLC methods for quality control has drastically increased. Six common cannabinoids of high medicinal interest cannabidiol (CBD), cannabidiolic acid (CBDA), cannabinol (CBN), Δ8-tetrahydrocannabinol (Δ8-THC), Δ9-tetrahydrocannabinol (Δ9-THC), and Δ9-tetrahydrocannabinolic acid (Δ9-THCA) were quantified using
the AZURA® HPLC plus system according to the monography of German Pharmacopeia [2]. Three different reversed phase columns were screened for robustness for the method verified in the subsequent step. Method verification took place using authorized medicinal cannabis flower samples available on the market. The substances were analysed with the help of chemical reference standards.

Technical Data

Application

Method RP Gradient
Sample CBD, CBDA, CBN. THC, THCA
Separation column Eurospher II C18P 4,6 x 150 mm, 3μm, 100 A

Separation conditions

Key words bediol, DAB, CBN, THC, DABA, THCA, cannabis, german pharmacopeia, cannabinoids, cannabis flowers, cannabis sativa L.
CAS number 13956-29-1, 1244-58-2, 521-35-7, 5957-75-5, 1972-08-3, 23978-85-0, 75-05-8, 64-17-5, 7664-38-2

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