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Analysis of Poly [(R)-3-hydroxybutyric acid] in chloroform using GPC and universal calibration

Application No.: VEV0084

Analysis of Poly [(R)-3-hydroxybutyric acid] in chloroform using GPC and universal calibration

Two Poly [(R)-3-hydroxybutyric acid] (PHB) standards (10 - and 500 kDa) were analysed in chloroform with SEC using universal calibration. For this purpose, a KNAUER system was used in combination with a linear GPC column. For the determination of the number average molecular mass (Mn) and the mass average molecular mass (Mw) as well as the poly dispersity (PD), polystyrene (PS) and polymethylmethacrylate (PMMA) calibrations were performed in chloroform. It could be shown that this system can be successfully used for the analysis of the low molecular weight standard. The molar mass of the 500 kDa standard, on the other hand, was greatly underestimated with this method, but this is probably related to the poor solubility or the sample preparation of the higher molecular weight standard.

Technical Data

Application details

Method HPLC
Mode GPC
Substances Poly [(R)-3-hydroxybutyric acid]
Key words PHB, Poly [(R)-3-hydroxybutyric acid], GPC, SEC, polymer, biopolymers, bioplastic, biodegradable, universal calibration, PS, PMMA, green SEC, green GPC, biobased plastic, polydispersity
CAS number 26063-00-3
Version Version 1/ 10/2021

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