Simulated moving bed chromatography (SMBC) was applied for the purification of xylitol from fermentation mashof a fed batch culture. This process enabled to purify xylitol with nearly 100 % purity and recovery. Thus, allowinglarge scale purification of xylitol from biological xylose-xylitol conversion process.
Overlay of analytical chromatograms of raffinate/all but xylitol (blue), extract/ xylitol (red) and waste (green) from the SMB process at the 6th cycle; 20 μL injection; Eurokat Ca 300 x 8 mm + pre-column; 10 μm particles, 0.5 mL/ min; 75°C
|Mode||Ion exclusion, Ligand exchange|
|Key words||SMB, SMBC, Valor Plus, purification, fermentation mash, xylitol, Eurokat, continuous purification, sugar substitute|
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