EN DE

Performance Verification (PV) Procedures for HPLC

A PV procedure is recommended for testing newly installed AZURA® HPLC systems as well as for regular monitoring of system performance.
This table gives an overview of the needed PV document, PV Standard and HPLC column for a specific AZURA system.

Back-pressure range Type of detection Flow cell path length Injection: Sample loop volume PV document Article number of PV standard Article number of HPLC column
    mm µl      
UHPLC systems
(max. 1000 bar)
UV, DAD 10 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-3 10BE181E2F
UV, DAD 10 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-2 10BE181E2F
UV, DAD 50 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-2 10BE181E2F
UV, DAD 50 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-1 10BE181E2F
FLD all 1 - 20 VPV-004: Analytical HPLC, FL detection A01262-2 10BE181E2F
FLD all 21 - 100 VPV-004: Analytical HPLC, FL detection A01262-3 10BE181E2F
HPLC Plus systems 
(max. 700 bar)
UV, DAD 10 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-4 15WE181E2J
UV, DAD 10 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-3 15WE181E2J
UV, DAD 50 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-3 15WE181E2J
UV, DAD 50 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-2 15WE181E2J
FLD all 1 - 20 VPV-004: Analytical HPLC, FL detection A01262-1 15WE181E2J
FLD all 21 - 100 VPV-004: Analytical HPLC, FL detection A01262-2 15WE181E2J
HPLC and UHPLC systems RID all all VPV-002: Analytical HPLC, RI detection A01261-1 05WE184E2J
Preparative  HPLC systems UV, DAD < 2 all VPV-007: Preparative HPLC, UV detection A01264-1 05JE181E2J
  UV, DAD > 2 all VPV-007: Preparative HPLC, UV detection A01264-2 05JE181E2J
  RID all all VPV-008: Preparative HPLC, RI detection A01265-1 05IE184E2J

Definition of PV Standards:

Article number butyl benzoate 136-60-7 pentyl benzoate 2049-96-9 uridin58-96-8 deoxyuridin 951-78-0 anthracene 120-12-7 Solvent Volume
  mg/mL mg/mL mg/mL mg/mL µg/mL   ml
A01260-4 0,50 0,50       ACN/H2O 8:2 1,2
A01260-3 0,10 0,10       ACN/H2O 8:2 1,2
A01260-2 0,02 0,02       ACN/H2O 8:2 1,2
A01260-1 0,01 0,01       ACN/H2O 8:2 1,2
A01261-1     1,00 1,00   H2O 1,2
A01261-2     0,10 0,10   H2O 1,2
A01262-1         0,030 ACN/H2O 8:2 1,2
A01262-2         0,006 ACN/H2O 8:2 1,2
A01262-3         0,003 ACN/H2O 8:2 1,2
A01264-1   2,00       ACN/H2O 75:25 10
A01264-2   0,5       ACN/H2O 75:25 10
A01265-1     40     H2O 10

Need a quote?

Or just more information about our products?

Please send us your request.
We are happy to answer your questions about our products and services.

 

+49 30 809727 0

Monday to Friday
 7 am- 5 pm CET

sales@knauer.net

Available for you anytime

Follow us