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Performance Verification (PV) Procedures for KNAUER Systems

A PV procedure is recommended for testing newly installed AZURA® systems as well as for regularly monitoring the system performance.
This table gives an overview of the needed PV document, PV standard and separation column for a specific AZURA system.
 

System classification Type of detection Flow cell path length Injection: Sample loop volume PV document Article number of PV standard Article number of separation column
    mm µl      
UHPLC systems
(max. 1000 bar)		 UV, DAD 10 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-3 10BE181E2F
UV, DAD 10 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-2 10BE181E2F
UV, DAD 50 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-2 10BE181E2F
UV, DAD 50 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-1 10BE181E2F
FLD all 1 - 20 VPV-004: Analytical HPLC, FL detection A01262-2 10BE181E2F
FLD all 21 - 100 VPV-004: Analytical HPLC, FL detection A01262-3 10BE181E2F
RID all all VPV-002: Analytical HPLC, RI detection A01261-1 05WE184E2J
HPLC Plus systems 
(max. 862 bar)		 UV, DAD 10 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-4 15WE181E2J
UV, DAD 10 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-3 15WE181E2J
UV, DAD 50 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-3 15WE181E2J
UV, DAD 50 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-2 15WE181E2J
FLD all 1 - 20 VPV-004: Analytical HPLC, FL detection A01262-1 15WE181E2J
FLD all 21 - 100 VPV-004: Analytical HPLC, FL detection A01262-2 15WE181E2J
RID all all VPV-002: Analytical HPLC, RI detection A01261-1 05WE184E2J
ECD in PAD mode n/a 20, 100 VPV-106: AZURA® systems with ECD A01132 n/a
ECD in DC mode n/a 20, 100 VPV-106: AZURA® systems with ECD A01273-2
A01273-3		 n/a
UV (normal phase) 10, 50 10, 20, 100 VPV-009: AZURA®
HPLC systems in normal phase mode		 n/a 15WE000E2J
RID (normal phase) all all VPV-009: AZURA®
HPLC systems in normal phase mode		 n/a 15WE000E2J
Preparative  HPLC systems UV, DAD ≤ 2 all VPV-007: Preparative HPLC, UV detection A01264-1 05JE181E2J
UV, DAD ≥ 2 all VPV-007: Preparative HPLC, UV detection A01264-2 05JE181E2J
RID all all VPV-008: Preparative HPLC, RI detection A01265-1 05IE184E2J
FPLC systems UV, DAD all all VPV-003: AZURA®
FPLC systems		 A01261-1 05WE184E2J

Definition of PV Standards:

Article number Butyl benzoate
136-60-7		 Pentyl benzoate
2049-96-9		 Uridin
8-96-8		 Deoxyuridin
951-78-0		 Anthracene
120-12-7		 Solvent Volume
  mg/mL mg/mL mg/mL mg/mL µg/mL   ml
A01260-4 0.50 0.50       ACN/H2O 8:2 1.2
A01260-3 0.10 0.10       ACN/H2O 8:2 1.2
A01260-2 0.02 0.02       ACN/H2O 8:2 1.2
A01260-1 0.01 0.01       ACN/H2O 8:2 1.2
A01261-1     1.00 1,00   H2O 1.2
A01261-2     0.10 0,10   H2O 1.2
A01262-1         0.030 ACN/H2O 8:2 1.2
A01262-2         0.006 ACN/H2O 8:2 1.2
A01262-3         0.003 ACN/H2O 8:2 1.2
A01264-1   2.00       ACN/H2O 75:25 10
A01264-2   0.50       ACN/H2O 75:25 10
A01265-1     40     H2O 10

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System Operation Qualification (OQ) Procedures for HPLC

The Operation Qualification includes extensive functionality tests of pump, autosampler and detector to prove that the system runs reliably within the documented specifications.

This table gives an overview of the available standards and equipment for a system OQ.
 

Article number Description Items included
A66310 Equipment for System OQ System OQ test document VOQ-SYS-01 Restriction capillary System OQ Kit of Caffeine Standards: Set 1
A66320 Equipment for System OQ System OQ Kit of Caffeine Standards: Set 2
A01269-10 System OQ Test Standard Set 1     System OQ Kit of Caffeine Standards: Set 1
A01269-20 System OQ Test Standard Set 2     System OQ Kit of Caffeine Standards: Set 2

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