Although time is relative, the total run time of a method is an important factor when it comes to high throughput analysis. Saving time means saving money. With the exemplary separation of compounds that are present in anti-epileptic drugs, the KNAUER AZURA UHPLC system equipped with the new binary AZURA P 8.1L pump, thanks to the low dispersion and operating pressure up to 1240 bar, allows to speed up your method and increase throughput preserving resolution.
Chromatogram at 0.2 ml/min, (1) caffeine, (2) PEMA, (3) ethosuximide, (4) primidone, (5) carbamazepine, (6) phenytoin
Chromatogram at 1.5 ml/min, (1) caffeine, (2) PEMA, (3) ethosuximide, (4) primidone, (5) carbamazepine, (6) phenytoin
Overlay of chromatograms at different flow rates, dark blue- 0.2 ml/ min, red – 0.6 ml/min, black – 1.2 ml/min, light blue – 1.5 ml/min
Standard deviation of retention time over 10 replicates for each flow rate
Normalized overlay of primidone (peak 4), dark blue- 0.2 ml/min, red – 0.6 ml/min, black – 1.2 ml/min, light blue – 1.5 ml/min
Van Deemter plots
| Method | UHPLC |
|---|---|
| Mode | RP |
| Substances | Caffeine, ethosuximide, primidone, carbamazepine, phenytoin, 2-ethyl-2-phenylmalonamide monohydrate |
| Key words | (U)HPLC, RP, AZURA, P 8.1L, anti-epileptic drugs, high throughput, analysis time, band broadening, extra column volume, symmetry, van Deemter, system volumes |
| CAS number | 80866-90-6, 58-08-2, 77-67-8, 125-33-7, 298-46-4, 57-41-0 |
| Version | version 1 06/2023 |
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