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Determination of six steviol glycosides using reversed phased HPLC and online SPE

Applikationsnr.: VFD0174

Determination of six steviol glycosides using reversed phased HPLC and online SPE

Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and can be used as natural sugar substitutes, because they have a far higher sweetening power than normal sucrose or glucose. The sweetness is estimated to be about 400 times higher. This gradient method provides a fast determination of six steviol glycosides using reversed phase HPLC and UV detection. Furthermore, an automated matrix reduction is achieved by online SPE (solid phase extraction), speeding up sample preparation and guaranteeing a high sample throughput.

Technische Daten

Applikation

Methode HPLC

Trennbedingungen

Modus RP, Online SPE
Substanzen steviol glycosides
Schlüsselwörter Stevia rebaudiana; steviol glycosides; rebaudioside A; stevioside; rebaudioside C; dulcoside A; rebaudioside B; steviolbioside; stevia sweetener; stevia; method transfer; Scale-Up; overload; HPLC; reversed phase; gradient; stevia sweetener; analytical; SPE; online SPE; automation; sample preparation; AZURA
CAS Nummer 58543-16-1, 57817-89-7, 63550-99-2, 64432-06-0, 58543-17-2, 41093-60-1

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