Oligonucleotides are highly important biopharmaceuticals with strongly increasing relevance. Due to the high similarity of impurities originating from the synthesis, however, purification can be challenging.

This seminar demonstrates a successful large scale purification of an oligonucleotide using anion exchange chromatography (AEX) as well as efficient quality control by applying ion-pairing reversed phase chromatography (IP-RP).

Dr. Ulrike Krop, KNAUER Wissenschaftliche Geräte GmbH
Dr. Daniel Eßer, YMC Europe GmbH

Content

• How to perform a large scale AEX purification: Design of preparative system and useful features
• Benefits and limitations using AEX for preparative purification
• What to consider for developing an IP-RP methods: selection of stationary phase, ion-pair reagent, and solvent conditions
• How to improve analytical results by utilising bioinert column hardware

Peptides have become one of the most important biopharmaceuticals. The high variability related to chain length and sequence of amino acids offers multiple pharmaceutical applications, and leads to specific demands regarding the production and purification.

This seminar therefore aims to provide the knowledge required for development of peptide purification methods based on case studies of up-to-date targets like a GLP-1 agonist and a peptide hormone.

Speakers
Julia Bartmann, YMC Europe GmbH
Dr. Yannick Krauke, KNAUER Wissenschaftliche Geräte GmbH

Content

• How to choose the ideal stationary phase: the most significant aspects to consider and the importance of extensive screening
• What to consider for method development: finding efficient conditions by appropriate selection of solvents, pH and buffers
• From overview to focused gradient: how to optimize a robust preparative method
• Linear scale-up: Understanding the principle, system design and sample collection

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