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Separation of Pyrimidine and Purine Bases in HILIC mode with AZURA Compact

Applikationsnr.: VBS0035J

Separation of Pyrimidine and Purine Bases in HILIC mode with AZURA Compact

The separation of compounds chosen among purine and pyrimidine bases was studied by using hydrophilic interaction chromatography (HILIC). The retention of the analytes was studied by varying ACN%, ammonium formate concentration, pH, and column temperature. The results obtained confirmed the elution order of nucleobases and nucleosides on their hydrophobicity.

Technische Daten

Applikation

Methode HPLC

Trennbedingungen

Modus HILIC
Substanzen 3,5- Di-tert-butyltoluene, Adenine, Cytosine, Uracil
Schlüsselwörter HILIC, AZURA Compact, Eurospher II HILIC, Purine, Pyrimidine
CAS Nummer 15181-11-1, 66-22-8, 73-24-5, 71-30-7

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