The purification of antibodies is generally performed with protein A column materials. Two prepacked protein A columns from two different suppliers were compared in their ability to purify immunoglobulin antibodies (IgG) from human plasma. Capacity and yield were compared and revealed no significant differences in the performances of the two investigated columns.
Chromatogram of antibody purification; Step 1: injection peak of human plasma and column washing; Step 2: elution peak of IgG from protein A column with buffer B; Step 3: column equilibration with buffer A
|IgG in human plasma
|IgG, antibodies, FPLC, proteinA – affinity, human plasma
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